rabbit anti lifr  (Proteintech)

Journal: Journal of Inflammation Research

Article Title: Association of Increased Nasal Fluids-Serum Concordance of Protein Profile with Prognosis in Nasal Polyps

doi: 10.2147/JIR.S567454

Figure Lengend Snippet: Expression validation of LIFR in CRSwNP. ( A ) Transcriptional levels of biomarkers (LIFR, LIF, IL-5, IL-13) in PR versus non-PR groups. ELISA quantification of LIFR concentrations in both ( B ) serum and ( C ) NF of PR patients compared to non-PR and control groups. ( D ) Representative images of H&E and LIFR immunohistochemical staining (Magnification ×400). ( E ) Quantification of LIFR protein expression intensity (n=6/group). ( F and G ) scRNA-seq analysis of CRSwNP tissues showing LIFR expression patterns across cell populations (left: cell type clusters; right: LIFR expression). ( H ) Immunofluorescence of LIFR (red) co-localization with vascular endothelial cells (CD31+, green) and nuclear staining (DAPI, blue) at ×400 magnifications (n=6/group). * P < 0.05, ** P < 0.01, **** P < 0.0001.

Article Snippet: After 1 h blocking with 10% BSA at room temperature, sections were incubated overnight at 4°C with primary antibodies, including neutrophil elastase (NE; Abcam #ab68672) for neutrophil quantification or LIFR (Origene #TA386958) for LIFR expression detection, followed by DAB substrate.

Techniques: Expressing, Biomarker Discovery, Enzyme-linked Immunosorbent Assay, Control, Immunohistochemical staining, Staining, Immunofluorescence

Journal: Cell Death Discovery

Article Title: Targeting metastasis-initiating cancer stem cells in gastric cancer with leukaemia inhibitory factor

doi: 10.1038/s41420-024-01839-1

Figure Lengend Snippet: A Representative images of the different types of TMA stained and analysed for LIFR expression (X20). Scale bars 100 µm. LIFR expression scored on TMA from GC patients. B and C Overall LIFR expression was analysed, and different comparisons were done: B Expression in Intestinal Metaplasia ( n = 25) and GC ( n = 146) were compared to non-tumorous tissue ( n = 81), C GC was separated into the Laurèn classification-based subtypes diffuse ( n = 33) and intestinal ( n = 113) and compared to expression in non-tumorous tissue. Values represented mean LIFR scores according to the following criteria: 0: no expression, 1: 1–20%, 2: 20–50%, 3: >50. D Progression-free survival curves showing patients’ survival percentage according to overall LIFR low expression (LIFR ≤ 1, n = 40) and high expression (LIFR > 1, n = 68). E Correlation analysis of CD44v3 and LIFR-membrane expression scores ( n = 133). F Expression scores of CD44v3 and LIFR in tumours having low CD44v3 (CD44v3 ≤ 1, n = 92) and high CD44v3 expression (CD44v3 ≥ 2, n = 42). G–I Progression-free survival curves showing survival percentage of GC patients (14 ≤ n ≤ 69) according to G CD44v3 expression in all patients independent of LIFR expression profile; H CD44v3 expression in patients having high LIFR expression; I CD44v3 expression in patients having low LIFR expression. Red bars represent high CD44v3 expression and black bars have low CD44v3 expression. J–L KMplot database analyses showing overall survival probability of GC patients (69 ≤ n ≤ 249) according to J ZEB1 expression in all patients independent of LIFR expression profile; K ZEB1 expression in patients having high LIFR expression; L ZEB1 expression in patients having low LIFR expression. Red bars represent high ZEB1 expression and black bars have low ZEB1 expression. * p < 0.05, ** p < 0.005, *** p < 0.0005 and **** p < 0.0001 vs. the conditions indicated by the bars, ANOVA, Wilcoxon paired t-test statistical analyses, paired t -test and log-rank test.

Article Snippet: Primary antibodies used were rabbit anti-LIFR (Abcam, Cambridge, UK) 1:200, anti-CD44 (BD Biosciences) 1:100 and anti-CD44v3 (R&D systems, clone 3G5) 1:4000.

Techniques: Staining, Expressing, Membrane

Journal: The Journal of international medical research

Article Title: Role and mechanism of leukemia inhibitory factor receptor in cervical cancer invasion and metastasis.

doi: 10.1177/03000605231182557

Figure Lengend Snippet: Figure 2. Comparison of the expression of Hippo–YAP signaling pathway-related proteins. LIFR, P-YAP, and P-TAZ protein expression were higher in the study group than in the control group, whereas YAP and TAZ protein expression was higher in the control group than in the study group. ***P < 0.001.

Article Snippet: Rabbit anti-human antibodies against LIFR (Cat. No. 17027T), GAPDH (Cat. No. 5174), P-YAP (Cat. No. 13008), and P-TAZ (Cat. No. 52420) were obtained from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Comparison, Expressing, Control